Persistence of DNA in Carcasses, Slime and Avian Feces May Affect Interpretation of Environmental DNA Data
نویسندگان
چکیده
The prevention of non-indigenous aquatic invasive species spreading into new areas is a goal of many resource managers. New techniques have been developed to survey for species that are difficult to capture with conventional gears that involve the detection of their DNA in water samples (eDNA). This technique is currently used to track the invasion of bigheaded carps (silver carp and bighead carp; Hypophthalmichthys molitrix and H. nobilis) in the Chicago Area Waterway System and Upper Mississippi River. In both systems DNA has been detected from silver carp without the capture of a live fish, which has led to some uncertainty about the source of the DNA. The potential contribution to eDNA by vectors and fomites has not been explored. Because barges move from areas with a high abundance of bigheaded carps to areas monitored for the potential presence of silver carp, we used juvenile silver carp to simulate the barge transport of dead bigheaded carp carcasses, slime residue, and predator feces to determine the potential of these sources to supply DNA to uninhabited waters where it could be detected and misinterpreted as indicative of the presence of live bigheaded carp. Our results indicate that all three vectors are feasible sources of detectable eDNA for at least one month after their deposition. This suggests that current monitoring programs must consider alternative vectors of DNA in the environment and consider alternative strategies to minimize the detection of DNA not directly released from live bigheaded carps.
منابع مشابه
P-94: Mouse Embryo Vitrification Cannot Effect on Global DNA Methylation in Preimplantation Stage
Background: Embryo vitrification was effectively used for assisted reproductive techniques. Despite the undeniable benefits of vitrification, cooling and warming stress, and cytotoxicity of cryoprotectant may affect the DNA methylation that have an important role in gene activation and silencing. In the present study effects of 2-cell embryo vitrification on DNA methylation in hatched blastocys...
متن کاملEpigenetic Modifications of Host Genes Induced by Bacterial Infection
Introduction: Epigenetic mechanisms regulate expression of the genome to generate various cell types during development or coordinate cellular responses to external stimulus. While epigenetics is of fundamental importance in eukaryotes, it plays a different role in bacteria. This article uncovers the most important recent data on how bacteria can alter epigenetic marks and can also contribute t...
متن کاملThe biometric and cytochrome oxidase subunit I (COI) gene sequence analysis of syngnathus abaster (Teleostei: Syngnathidae) in Caspian sea
The black-striped pipefish, Syngnathus abaster, is a species of the Syngnathidae family. This study provides data on the morphometric, meristic and genetic structuring of the Caspian Sea pipefish, Syngnathus abaster. Eight morphometric characteristics based on total length and five morphometric characteristics based on standard length were analyzed. A total of 50 specimens were collected in bra...
متن کاملImmune efficacy of OmpH and OmpA DNA vaccines against avian Pasteurella multocida
Avian Pasteurella multocida is an agent of fowl cholera. The protective effect achieved through orthodoxvaccines is not ideal. The research on novel vaccines against avian Pasteurella multocida is imperative. Inthis study, the genes encoding outer membrane protein H and A (OmpH and OmpA) were cloned into theeukaryotic expression vector pcDNA3.1(+) and the recombinant plasmids, namely DNA vaccin...
متن کامل